Life Bio Fil is a natural biological filter medium. Selected strains of bacteria soothes maturation through aid of nitrification process. The filling increases filtration efficiency in existing aquariums and is an excellent filter media for newly estabilish ones. The main advantage is live bacteria as an ingredient of Life Bio Fill. This means that product works immediately and there is no need to wait until the inhabitation of bacteria. Life bio fil should be placed in the sump in a good water flow. 1000 ml (33.81 fl oz) medium should be used in an aquarium with a capacity of 400L(105 US gal.) We recommend to replace 10 % – 20 % of the filling every 6 weeks.